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Evaluation of antibody specificity by western blot. Testing of α- Pl CBP49 mAb1 with eight different strains of P. larvae ERIC I ( A ) and eight different strains of P. larvae ERIC II (B); native Pl CBP49 served as a positive control. Testing of α-SplA mAb2 with eight different strains of P. larvae ERIC I ( C ) and eight different strains of P. larvae ERIC II ( D ); recSplA served as a positive control. The Precision Plus Protein <t>WesternC</t> <t>Blotting</t> Standard (Bio-Rad, Feldkirchen, Germany) served as a molecular weight marker for all blots. The original blots in full size are presented in supplementary Figure S2.
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Evaluation of antibody specificity by western blot. Testing of α- Pl CBP49 mAb1 with eight different strains of P. larvae ERIC I ( A ) and eight different strains of P. larvae ERIC II (B); native Pl CBP49 served as a positive control. Testing of α-SplA mAb2 with eight different strains of P. larvae ERIC I ( C ) and eight different strains of P. larvae ERIC II ( D ); recSplA served as a positive control. The Precision Plus Protein WesternC Blotting Standard (Bio-Rad, Feldkirchen, Germany) served as a molecular weight marker for all blots. The original blots in full size are presented in supplementary Figure S2.

Journal: Scientific Reports

Article Title: Immunoassays for the detection and differentiation of Paenibacillus larvae , the etiological agent of American foulbrood (AFB) in honey bees

doi: 10.1038/s41598-026-35590-7

Figure Lengend Snippet: Evaluation of antibody specificity by western blot. Testing of α- Pl CBP49 mAb1 with eight different strains of P. larvae ERIC I ( A ) and eight different strains of P. larvae ERIC II (B); native Pl CBP49 served as a positive control. Testing of α-SplA mAb2 with eight different strains of P. larvae ERIC I ( C ) and eight different strains of P. larvae ERIC II ( D ); recSplA served as a positive control. The Precision Plus Protein WesternC Blotting Standard (Bio-Rad, Feldkirchen, Germany) served as a molecular weight marker for all blots. The original blots in full size are presented in supplementary Figure S2.

Article Snippet: The Precision Plus Protein WesternC Blotting Standard (Bio-Rad, Feldkirchen, Germany) served as a molecular weight marker for all blots.

Techniques: Western Blot, Positive Control, Molecular Weight, Marker